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e cadherin immunofluorescence staining  (Proteintech)


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    Proteintech e cadherin immunofluorescence staining
    E Cadherin Immunofluorescence Staining, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 2942 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/e+cadherin+immunofluorescence+staining/pm40825974-376-12-16?v=Proteintech
    Average 96 stars, based on 2942 article reviews
    e cadherin immunofluorescence staining - by Bioz Stars, 2026-08
    96/100 stars

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    Fig. 1 Stemness and EMT-like phenotypes of trastuzumab-resistant gastric cancer. (a) NCI-N87R cells had significantly larger sphere size and comprised higher cell counts of spheres larger than 50 μm compared with NCI-N87 cells. (b) E-cadherin was significantly downregulated in NCI-N87R cells, as confirmed by <t>immunofluorescence,</t> compared with parental cells. Stem cell markers including CD44s, CD54, BMI1, Oct4, Vimentin and Snail were significantly upregulated in NCI-N87R cells compared with parental cells, according to Western blot analysis. *P < 0.05, **P < 0.01, ***P < 0.005
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    Macranthoidin B downregulated epithelial-mesenchymal transition and COX-2/PGE 2 pathway in endometriotic tissues. (A–D) The mRNA levels of Cdh1 , Cdh2 , Vim , Twist , Slug , Snail , and Zeb1/2 were measured by RT-qPCR. (E–G) The protein levels of E-cadherin, N-cadherin, and Vimentin were detected by Western blotting assay. (H) Detection of E-caderin and Vimentin in ectopic tissue by immunofluorescence assay. (I) The gene expression of Ptgs2 and Ptges were tested by RT-qPCR. (J–L) The contents of COX-2 and PGE 2 were analyzed by Western blotting and ELISA assays. # P < 0.05 to control, ## P < 0.01 to control, * P < 0.05 to EMS ** P < 0.01 to EMS. Columns, mean (n = 3). Bars, SD. Scale bar = 100 μm. EMS, endometriosis.

    Journal: Frontiers in Pharmacology

    Article Title: Macranthoidin B restrains the epithelial-mesenchymal transition through COX-2/PGE 2 pathway in endometriosis

    doi: 10.3389/fphar.2024.1492098

    Figure Lengend Snippet: Macranthoidin B downregulated epithelial-mesenchymal transition and COX-2/PGE 2 pathway in endometriotic tissues. (A–D) The mRNA levels of Cdh1 , Cdh2 , Vim , Twist , Slug , Snail , and Zeb1/2 were measured by RT-qPCR. (E–G) The protein levels of E-cadherin, N-cadherin, and Vimentin were detected by Western blotting assay. (H) Detection of E-caderin and Vimentin in ectopic tissue by immunofluorescence assay. (I) The gene expression of Ptgs2 and Ptges were tested by RT-qPCR. (J–L) The contents of COX-2 and PGE 2 were analyzed by Western blotting and ELISA assays. # P < 0.05 to control, ## P < 0.01 to control, * P < 0.05 to EMS ** P < 0.01 to EMS. Columns, mean (n = 3). Bars, SD. Scale bar = 100 μm. EMS, endometriosis.

    Article Snippet: For immunofluorescence staining, rabbit anti-E-cadherin or anti-Vimentin antibodies (1:200 dilution; Proteintech, China) were applied to the endometrial tissue.

    Techniques: Quantitative RT-PCR, Western Blot, Immunofluorescence, Gene Expression, Enzyme-linked Immunosorbent Assay, Control

    Fig. 1 Stemness and EMT-like phenotypes of trastuzumab-resistant gastric cancer. (a) NCI-N87R cells had significantly larger sphere size and comprised higher cell counts of spheres larger than 50 μm compared with NCI-N87 cells. (b) E-cadherin was significantly downregulated in NCI-N87R cells, as confirmed by immunofluorescence, compared with parental cells. Stem cell markers including CD44s, CD54, BMI1, Oct4, Vimentin and Snail were significantly upregulated in NCI-N87R cells compared with parental cells, according to Western blot analysis. *P < 0.05, **P < 0.01, ***P < 0.005

    Journal: BMC cancer

    Article Title: Wnt/β-catenin pathway is a key signaling pathway to trastuzumab resistance in gastric cancer cells.

    doi: 10.1186/s12885-023-11447-4

    Figure Lengend Snippet: Fig. 1 Stemness and EMT-like phenotypes of trastuzumab-resistant gastric cancer. (a) NCI-N87R cells had significantly larger sphere size and comprised higher cell counts of spheres larger than 50 μm compared with NCI-N87 cells. (b) E-cadherin was significantly downregulated in NCI-N87R cells, as confirmed by immunofluorescence, compared with parental cells. Stem cell markers including CD44s, CD54, BMI1, Oct4, Vimentin and Snail were significantly upregulated in NCI-N87R cells compared with parental cells, according to Western blot analysis. *P < 0.05, **P < 0.01, ***P < 0.005

    Article Snippet: Immunofluorescence staining of E-Cadherin and β-Catenin Rabbit monoclonal antibodies against E-cadherin (1:1,000; #sc-7870; Santa Cruz Biotechnology, Dallas, TX, USA) and β-catenin (1:50; #sc-7199; Santa Cruz Biotechnology) were used to label the NCI-N87 and NCI-N87R cells.

    Techniques: Immunofluorescence, Western Blot

    Fig. 2 Increased activity of Wnt signaling pathway of tastuzumab-resistant gastric cancer cells. (a) NCI-N87R cells showed significantly higher activity of the Wnt signaling pathway compared with that of parental cells. (b) β-catenin was significantly upregulated in NCI-N87R cells compared with parental cells, as observed on immunofluorescence staining. P < 0.05, **P < 0.01, ***P < 0.005

    Journal: BMC cancer

    Article Title: Wnt/β-catenin pathway is a key signaling pathway to trastuzumab resistance in gastric cancer cells.

    doi: 10.1186/s12885-023-11447-4

    Figure Lengend Snippet: Fig. 2 Increased activity of Wnt signaling pathway of tastuzumab-resistant gastric cancer cells. (a) NCI-N87R cells showed significantly higher activity of the Wnt signaling pathway compared with that of parental cells. (b) β-catenin was significantly upregulated in NCI-N87R cells compared with parental cells, as observed on immunofluorescence staining. P < 0.05, **P < 0.01, ***P < 0.005

    Article Snippet: Immunofluorescence staining of E-Cadherin and β-Catenin Rabbit monoclonal antibodies against E-cadherin (1:1,000; #sc-7870; Santa Cruz Biotechnology, Dallas, TX, USA) and β-catenin (1:50; #sc-7199; Santa Cruz Biotechnology) were used to label the NCI-N87 and NCI-N87R cells.

    Techniques: Activity Assay, Immunofluorescence, Staining

    Fig. 3 Increased activity of Wnt signaling pathway of gastric cancer cells incubated in Wnt3a-conditioned medium. (a) NCI-N87_WNT cells showed significantly increased activity of the Wnt signaling pathway. (b) Immunofluorescence staining revealed that NCI-N87_WNT cells had markedly higher levels of β-catenin expression than NCI-N87 cells. (c) E-cadherin was downregulated in NCI-N87_WNT cells, and this was similar in NCI-N87R cells. *P < 0.05, **P < 0.01, ***P < 0.005

    Journal: BMC cancer

    Article Title: Wnt/β-catenin pathway is a key signaling pathway to trastuzumab resistance in gastric cancer cells.

    doi: 10.1186/s12885-023-11447-4

    Figure Lengend Snippet: Fig. 3 Increased activity of Wnt signaling pathway of gastric cancer cells incubated in Wnt3a-conditioned medium. (a) NCI-N87_WNT cells showed significantly increased activity of the Wnt signaling pathway. (b) Immunofluorescence staining revealed that NCI-N87_WNT cells had markedly higher levels of β-catenin expression than NCI-N87 cells. (c) E-cadherin was downregulated in NCI-N87_WNT cells, and this was similar in NCI-N87R cells. *P < 0.05, **P < 0.01, ***P < 0.005

    Article Snippet: Immunofluorescence staining of E-Cadherin and β-Catenin Rabbit monoclonal antibodies against E-cadherin (1:1,000; #sc-7870; Santa Cruz Biotechnology, Dallas, TX, USA) and β-catenin (1:50; #sc-7199; Santa Cruz Biotechnology) were used to label the NCI-N87 and NCI-N87R cells.

    Techniques: Activity Assay, Incubation, Immunofluorescence, Staining, Expressing